There are three different methods for the inoculation of the packaging material: Normally the spore suspensions are washed 3-4 times and centrifuged in order to remove any kind of foreign matter. Regarding spores of moulds, they need to be free from organic material coming from cultivation.Spore dilutions need to be prepared in pure sterile water.The spores need to be prepared in a water suspension free from salts and organic material that could provide a residue during drying.For spore suspensions, there should be different, independent suppliers available.Spores of bacteria's or moulds should be from a germ which is listed in an official type culture collection and commercially available.All 90,000 bottles are visually inspected. Completely filled bottles with liquid nitrogen treatment are stored at constant temperature of 25 to 30☌ for 21 days.Half filled bottles with liquid nitrogen treatment are stored at constant temperature of 25 to 30☌ for 14 days.Half filled or completely filled bottles are stacked on pallets and stored in a secured place at constant temperature of 25 to 30☌ for 14 days.Additionally 3,000 bottles per run are checked for pH level. Defective bottles change their size or their shape (gas production or vacuum generation). In case of non-transparent bottles, 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32☌.Additionally 1,000 bottles per run are checked for pH. In case of turbid product in clear bottles, all 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32☌.In case of culture medium or clear product in clear bottles, all 30,000 bottles are visually inspected after 7 days of incubation at a temperature of 30°-32☌.
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